Figure 3.

FUS^FL harbors distinct low-complexity disordered domains (LCDs) that individually undergo crowding-mediated liquid–liquid phase separation (LLPS). (a) Domain architecture of FUS; two distinct disordered domains are highlighted. The N-terminal FUS LCD has prion-like sequence features (FUS^PrD) and C-terminal LCD is enriched in RGG-repeats (FUS^RGG). The disorder prediction scores (using VSL2 algorithm [60]) are also shown in the bottom panel. (b,c) Effect of PEG on the LLPS of prion-like LCD and arginine-rich polycationic LCD (R-rich LCD). Shown here are the phase diagrams of FUS^PrD and FUS^RGG with PEG8000, respectively. The dotted lines indicate the liquid–liquid coexistence boundaries.