Fig. 4.

NELL-1 isoforms do not significantly induce cellular senescence associated gene expression among aged BMSC. Using BMSC derived from 14-month-old animals, lentiviral overexpression of NELL-1 isoforms was performed, including NELL-1810 and NELL-1570. In all cases, control cells were treated with a lentivirus expressing GFP (FG12). Gene expression analysis by qRT-PCR among BMSC derived from aged (14 months) mice. With the exception of p53 induction (D), no significant increase was observed across gene markers of senescence, including (A) p16, (B) p21, or (C) p27. Similar findings were observed among adult-derived BMSC. *P < 0.05.