Figure 4.

JQ1 abrogation of photostress-enhanced glioblastoma cell invasiveness. U251 cells at ~60% confluency were pre-incubated with 1 mM ALA. After PDT irradiation (1 J/cm²), these cells, along with dark controls, were harvested and assessed for invasiveness in the absence vs. presence of 0.3 µM JQ1, 0.3 µM JQ1(−), 5 µM Bay11, or 25 µM 1400W. DMSO served as a vehicle control for JQ1 and JQ1(−). Cells crossing Matrigel-infused filters after an incubation period of 24 h were pelleted and quantified by CCK-8 assay. Plotted invasion values are means ± SEM (n = 3); * p < 0.001 vs. PDT; ** p < 0.01 vs. PDT. Reproduced from Reference [61], with permission.