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. 2019 Feb 14;11(2):220. doi: 10.3390/cancers11020220

Figure 2.

Figure 2

Stellettin B inhibits migration and invasion of glioblastoma (GBM) cells. (A) Scratch wound healing assay on GBM8401 cells treated with 0, 0.5, 1, 2.5, or 5 μM stellettin B for 6 or 24 h. Scale bar = 200 µm. (B) Distance of cell migration was quantified using SPOT Imaging Microscopy Imaging Software. The result is representative of three separate experiments and is presented as mean ± SD (n = 3). * p < 0.05 comparing starting time. (C) Cell migration was measured using a transwell chamber (8 µm pore). U87MG and GBM8401 cells were treated with 0, 1, 5, or 10 µM stellettin B for 24 h. Migrated cells were stained with Giemsa solution, magnification 200×. (D) The number of migrated cells on the underside of the transwell insert was counted per file. Data are presented as mean ± SD (n = 3). * p < 0.05 relative to controls.