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. 2019 Feb 14;11(2):220. doi: 10.3390/cancers11020220

Figure 5.

Figure 5

Stellettin B inhibits Stat3/HIF-1α in VEGF expression and secretion in glioblastoma cells. (A) U87MG and GBM8401 cells were treated with 0, 1, 5, 10, or 25 μM stellettin B for 24 h. Western blot analysis was performed to study the expression of pStat3, Stat3, HIF-1α, and VEGF. β-Actin was used as a loading control. pStat3/Stat3, HIF-1α/ β-Actin, and VEGF/ β-Actin were quantified. Values are expressed as the mean ± SD (n = 3). * p < 0.05 relative to controls. (B) VEGF secretion in U87MG and GBM8401 cells after 0, 1, 5, 10, or 25 μM stellettin B treatment for 24 h. Secreted VEGF was measured using the Quantikine® Human VEGF immunoassay kit. Values are expressed as the mean ± SD (n = 4). * p < 0.05 relative to controls.