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. 2019 Jan 31;11(2):159. doi: 10.3390/cancers11020159

Figure 4.

Figure 4

2-Deoxy-D-glucose (2-DG) induces ER stress in GSCs in a dose-dependent manner. (A) CellTiter 96® AQueous One Solution Cell Proliferation (Promega, Madison, WI, USA) assay of Glio9, Glio11, and Glio14 treated with increasing doses of 2-DG for 72 h. Viability is reported as mean ± SEM. Data represent average values of at least three independent experiments. (B) Western blot analysis for ER stress markers (GRP78, GRP94, and CHOP) in GSC lines treated with 0.0, 0.25, 0.5, 1.0, 2.0, and 4.0 mM of 2-DG for 24 h. (C) Western blot analysis of GSCs treated with 2-DG and/or mannose to determine mechanism of rescue from ER stress.