Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 22;11(2):127. doi: 10.3390/cancers11020127

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

BCL2-associated transcription factor 1 (BCLAF1), BCL2 interacting protein 1 (BNIP1), or DEAD-box helicase 5 (DDX5) interacted with TRIB3 within radioresistant MDA-MB-231 cells. (A) Immunoprecipitation and mass spectrometry analysis were performed for total proteins from parental (P) or radioresistant (RR) MDA-MB-231 cells as described in the Materials and Methods section. The heatmap of identified proteins was created using the ClustVis web tool (https://biit.cs.ut.ee/clustvis/). (B) The expression of DDX5, BCLAF1, and BNIP1 between parental or radioresistant MDA-MB-231 cells was determined using Western blot. (C) The interaction between TRIB3 and DDX5, BCLAF1, or BNIP1 was determined by immunoprecipitation with anti-TRIB3 antibody and immunoblotting with antibodies of DDX5, BCLAF1, or BNIP1. (D) The expression of BCLAF1, BNIP1, or DDX5 in TRIB3 knockdown (KD1 or KD2) by lentiviral transduction of TRIB3 specific shRNAs or in sh-LacZ transduced (control) 231-RR cells was determined by Western blot. (E) The prognostic analysis of BCLAF1 or DDX5 in overall survival among breast cancer patients was obtained from The Human Protein Atlas website (https://www.proteinatlas.org/).