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. 2019 Jan 30;9(2):50. doi: 10.3390/biom9020050

Figure 1.

Figure 1

Effects of heat shock protein 27 (HSP27) phosphorylation on shear-stress-induced HSP27 polar distribution. (A) Fluorescence images of HeLa cells transfected with HSP27-Ypet (control) and HSP27-3A-Ypet (3A) plasmids before and after 30 min of 5, 20, and 40 dyn/cm2 shear stress stimulations. (B,C) The percentage of HSP27-Ypet and HSP27-3A-Ypet fluorescence intensity comparison of upstream to downstream in the control and 3A groups, respectively (* p < 0.05 when comparing to the upstream, with values taken from the 30 min time point). (D,E) The 3D distribution map of HSP27-Ypet and HSP27-3A-Ypet under 5, 20, and 40 dyn/cm2 shear stresses in the control and 3A groups, respectively. The number of repeated experiments (n) for 5, 20, and 40 dyn/cm2 in the control group was 14, 24, and 9, respectively, and n for the 3A group was 8, 9, and 11 for 5, 20, and 40 dyn/cm2, respectively).