Effect of HSP27 phosphorylation on shear-stress-induced HSP27 depolymerization. (A) FRET ratio images of the control, 3A, and KRIBB3 groups before and after 20 dyn/cm2 of shear stress stimulation. (B) The fluorescence resonance energy transfer (FRET) ratio in the control, 3A, and KRIBB3 groups under different magnitude of shear stress (SS, from 0 min) after normalization. (C) The FRET ratio comparison in the control, 3A, and KRIBB3 groups (* p < 0.05 when comparing to the control group, with values taken from the 30 min time point). n for 5, 20, and 40 dyn/cm2 in the control group is 24, 12, and 15; for the 3A group it is 19, 15 and 13; and in the KRIBB3 group it is 17, 20, and 21, respectively.