Table 2.
Experimental matrix and results obtained from the MAE.
| Treatment Number | MAE (min) |
pH | Solvent Concentration (Ethanol) (%) | Solvent-to-Seed Ratio (X4:1) | MAE | |
|---|---|---|---|---|---|---|
| Polyphenols (mg GA/g Seed) n = 3 |
Bixin (%) n = 3 |
|||||
| 1 | 2.5 | 7.5 | 73.0 | 10.0 | 2.83 ± 0.04 | 0.13 ± 0.00 |
| 2 | 2.5 | 4.0 | 50.0 | 6.0 | 1.53 ± 0.22 | 0.03 ± 0.00 |
| 3 | 2.5 | 7.5 | 50.0 | 10.0 | 2.85 ± 0.19 | 0.05 ± 0.00 |
| 4 | 5.0 | 4.0 | 96.0 | 6.0 | 1.96 ± 0.06 | 0.33 ± 0.04 |
| 5 | 2.5 | 7.5 | 73.0 | 6.0 | 1.96 ± 0.15 | 0.05 ± 0.01 |
| 6 | 5.0 | 11.0 | 73.0 | 6.0 | 2.01 ± 0.08 | 0.13 ± 0.02 |
| 7 | 5.0 | 11.0 | 73.0 | 6.0 | 1.35 ± 0.05 | 0.32 ± 0.01 |
| 8 | 2.5 | 11.0 | 96.0 | 6.0 | 3.94 ± 0.00 | 0.51 ± 0.01 |
| 9 | 5.0 | 7.5 | 50.0 | 6.0 | 3.79± 0.07 | 0.04 ± 0.01 |
| 10 | 2.5 | 7.5 | 50.0 | 2.0 | 0.53 ± 0.08 | 0.27 ± 0.03 |
| 11 | 2.5 | 4.0 | 73.0 | 6.0 | 4.16 ± 0.24 | 0.19 ± 0.00 |
| 12 | 2.5 | 4.0 | 73.0 | 2.0 | 0.83 ± 0.06 | 0.12 ± 0.01 |
| 13 | 2.5 | 7.5 | 73.0 | 6.0 | 1.89 ± 0.14 | 0.05 ± 0.01 |
| 14 | 2.5 | 7.5 | 96.0 | 6.0 | 1.72 ± 0.15 | 0.19 ± 0.00 |
| 15 | 2.5 | 11.0 | 73.0 | 10.0 | 3.25 ± 0.57 | 0.04 ± 0.01 |
| 16 | 2.5 | 11.0 | 50.0 | 6.0 | 4.36 ± 0.04 | 0.03 ± 0.00 |
| 17 | 2.5 | 7.5 | 96.0 | 6.0 | 2.43 ± 0.01 | 0.58 ± 0.00 |
| 18 | 2.5 | 7.5 | 96.0 | 2.0 | 0.66 ± 0.01 | 0.38 ± 0.03 |
| 19 | 0.0 | 7.5 | 73.0 | 6.0 | 1.96 ± 0.08 | 0.06 ± 0.01 |
| 20 | 5.0 | 7.5 | 96.0 | 10.0 | 2.10 ± 0.01 | 0.13 ± 0.00 |
| 21 | 2.5 | 7.5 | 73.0 | 2.0 | 0.70 ± 0.01 | 0.07 ± 0.02 |
| 22 | 0.0 | 7.5 | 73.0 | 6.0 | 1.90 ± 0.23 | 0.04 ± 0.00 |
| 23 | 2.5 | 11.0 | 73.0 | 2.0 | 0.68 ± 0.03 | 0.17 ± 0.01 |
| 24 | 0.0 | 7.5 | 73.0 | 10.0 | 2.15 ± 0.16 | 0.10 ± 0.01 |
| 25 | 2.5 | 4.0 | 73.0 | 6.0 | 2.28 ± 0.01 | 0.12 ± 0.03 |
| 26 | 0.0 | 7.5 | 73.0 | 6.0 | 2.02 ± 0.03 | 0.08 ± 0.00 |
| 27 | 0.0 | 7.5 | 50.0 | 6.0 | 3.90 ± 0.30 | 0.08 ± 0.00 |
| 28 | 2.5 | 7.5 | 73.0 | 2.0 | 1.57 ± 0.30 | 0.25 ± 0.01 |
| 29 | 0.0 | 4.0 | 73.0 | 10.0 | 1.96 ± 0.15 | 0.33 ± 0.01 |
| 30 | 5.0 | 7.5 | 73.0 | 6.0 | 1.91 ± 0.18 | 0.06 ± 0.02 |
Values are expressed as mean ± standard deviation (n = 3). MAE: microwave-assisted extraction, GA: gallic acid.