Table 3.

ANOVA for the response variables in the MAE.

Variable	Polyphenols (mg GA/g Seed)	Bixin (%)
	p-Value	p-Value
Model	<0.0001	<0.0001
X1, treatment time (min)	<0.0001	<0.0001
X2, pH	0.532	0.112
X3, solvent concentration (%)	<0.0001	<0.0001
X4, solvent-to-seed ratio (X4:1)	<0.0001	0.677
X2X3	0.001	˃0.050
X2X4	0.044	0.001
X3X4	˃0.050	0.004
X2X2	˃0.050	<0.0001
X4X4	<0.0001	˃0.050
X1X1	<0.0001	<0.0001
Lack of fit	0.0405	0.137
r2	0.931	0.914
r2-adj	0.901	0.875