Figure 2.

In vivo imaging demonstrates an increase in [18F]-GE180 uptake after cuprizone-intoxication. (A) The definition of VOIs (volumes of interest), demonstrated in a mouse brain MRI atlas in the coronal slides: medial corpus callosum (2.2 mm³), lateral corpus callosum (2.9 mm³), caudoputamen (4.4 mm³), thalamus (4.4 mm³), hippocampus (4.4 mm³), and cortex (6.7 mm³). (B) The quantification of radioligand uptake in the control animals (n = 4) and after a 5-week cuprizone intoxication period (n = 4). (C) Schematic illustration of the measured areas and cumulative heat map illustrating radioligand uptake in cuprizone-treated mice. The color codes demonstrate the standardized radioligand uptake levels relative to the controls (Z-scores; left column), and the normalized uptake in cuprizone-treated (middle column) and control (right column) mice. Cx (cortex); CC (corpus callosum); HC (hippocampus); Th (thalamus); CP (caudoputamen). Differences were determined using Mann–Whitney tests. * p  <  0.05, ** p  <  0.01, and *** p  <  0.001.