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. 2019 Feb 8;8(2):134. doi: 10.3390/cells8020134

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© 2019 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Positive reaction of MKs for endothelin-1 (ET-1), SPARC, and hepatocyte growth factor (HGF) in the bone marrow of femurs of control (CTR) and bone metastasis-bearing mice (ME). Representative images are shown, and three serial sections were analysed (n = 3). Semi-quantitative analysis of MK immunostaining is given below each panel: 4+ denotes very strong staining; 3+ strong staining; 2+ moderate staining; and 1+ weak staining. Statistical analysis is reported in supplementary materials (Table S1B). me: bone metastasis; bm: bone marrow; bo: bone. Scale bar = 20 μm.