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. 2019 Feb 23;11(2):261. doi: 10.3390/cancers11020261

Figure 1.

Figure 1

Galectin-1 expression decreases during osteoclast differentiation. (A) GSEA representing the distribution of the carbohydrate gene set in monocytes/macrophages versus osteoclasts (OCLs) and demonstrating that OCL precursors are higher enriched in carbohydrate binding proteins compared to mature OCLs. (B) Expression data set sorted by correlation with phenotype, the corresponding heat map, the gene tags and the leading-edge subset. Genes are ranked based on the correlation between their expression and the class distinction. (C) qPCR analyses of gal-1, including western blot analysis and OCL reference gene (NFATc1, cathepsin K, TRAP) expression in RAW-264.7-derived osteoclast cultures (MO: monocytes; OCL: osteoclasts; αTUB: α-tubulin). Data are representative of three (n = 3) biologically independent experiments and represented as mean +/− standard error. * p < 0.05; ** p < 0.01; *** p < 0.001. (D) Localization of gal-1 in mononuclear precursors (arrow) and mature OCLs (arrow head) (top to bottom: nucleus; actin, galectin-1, merge). (magnification: 60×) Representative images out of three (n = 3) independent experiments are shown.