Skip to main content
. 2019 Feb 23;11(2):261. doi: 10.3390/cancers11020261

Figure 2.

Figure 2

Loss of gal-1 enhances bone matrix resorption by osteoclasts. (A) Representative images of TRAP-stained primary (left) wild-type and (centre) gal-1−/−-derived osteoclast cultures. Quantification (right) of osteoclast number per well. (scale: 100 µm) (B) Resorbed matrix and quantification of the resorbed area. (C) Real-time PCR of osteoclast differentiation markers in mature osteoclasts derived from wild-type and gal-1−/− mice versus monocyte cultures (dotted line) (n.d.: not detected). From left to right: galectin-1 (LGALS1), NFATc1, cathepsin K (CTSK), TRAP, Integrin αv (ITGαv) and integrin β3 (ITGβ3). Significance level versus monocyte cultures. All data are representative of three (n = 3) biologically independent experiments and represented as mean +/− standard error. ## p < 0.05; ### p < 0.001; * p < 0.05; *** p < 0.001.