Figure 6.

In vivo evaluation of Ad5 and Ad5/3 replication in pigs. (a) Pigs were injected with a single dose of Ad5∆E3-Luc or Ad5/3∆E3-Luc at 3 × 10¹² vp via ear vein. Liver biopsies were performed at days 1, 2, and 4 post infection, primary organs were harvested 7-days post infection. (b) Viral DNA was present in lungs and spleen, but negligible copy numbers were measured in liver samples. Viral DNA copy numbers correlated well with replication dependent Luc expression. (c) Luc activity was detected in lungs and spleen. No significant replication-dependent Luc expression was observed in liver tissues.