Table 1.
Liquid chromatography-mass spectrometry analysis: principal identified peaks and relative abundance a.
| Peak Nr. | Ret Time (min) | [M-H]− m/z | [M+H]+ m/z | Other Related m/z Peaks | Relative Abundance | Putative Identity | Ref. | |
|---|---|---|---|---|---|---|---|---|
| T24/TN | T26/TN | |||||||
| 1 | 0.5 | 175.0 | 115 (M-C2H4O2) 215 (M+K) 199 (M+Na) |
4.46 | 2.62 | Ascorbic acid b | ||
| 2 | 4.4 | 193.0 | 195.0 | 177 (M-OH) | 1.59 | 0.99 | Ferulic acid b | |
| 3 | 4.9 | 787.2 | 655.1 (M-apiose-H) 331 (patuletin -H) |
3.00 | 1.14 | Patuletin-3-glucosyl-(1→6) [apiosyl(1→2)] glucoside | [32,33] | |
| 4 | 5.2 | 639.2 | 641.2 | 611.2 (M- 2H2O) 663.2 (M+Na) |
4.24 | 2.78 | Flavonol diglycoside | [34] |
| 5 | 5.5 | 481.3 | 463.3 (M-OH) 445.3 (M-2OH) 427.3 (M-3OH) |
2.48 | 0.89 | 20-hydroxyecdysone | [35] | |
| 6 | 6 | 429.3 | 431.3 | 453.3 (M+Na) | 2.34 | 1.32 | α-tocopherol | [36] |
| 7 | 6.1 | 535.4 | 537.4 | 2.85 | 0.87 | Jaceidin glucuronide | [33] | |
| 8 | 6.2 | 521.1 | 523.1 | 2.38 | 1.40 | Spinatoside | [32,33] | |
| 9 | 6.4 | 519.1 | 521.1 | 309 (M-glucuronide) | 2.77 | 1.15 | 5,3′,4′-Trihydroxy-3-methoxy-6:7-methylendioxyflavone-4′-glucuronide | [32,33] |
| 10 | 6.5 | 533.1 | 535.1 | 557.1 (M+Na) 505.1 (M-MeO) |
2.59 | 0.94 | 5,4′-dihydroxy-3,3′-dimethoxy-6:7-methylendioxyflavone-4′-glucuronide | [32,33] |
a Principal identified peaks and relative abundance referred to the control and hypothesis about chemical identity. T24 and T26: sampling times after 24 h of hypoxia (T24) and further 2 h of re-oxygenation (T26). TN relative normoxic control. b Compared with the standard compound.