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. 2019 Feb 21;8(2):187. doi: 10.3390/cells8020187

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Qualitative and quantitative evaluation of LDs in HeLa cells transiently transfected with ATGL wild-type and mutant proteins. After incubation for 18 h with oleic acid (OA) (400 μM) complexed to bovine serum albumin (BSA) (6:1 molar ratio), HeLa cells were transiently transfected with either phosphor (p) enhanced GFP (EGFP; A), pATGL-EGFP (B), pATGL(D166G)-EGFP (C), or pATGL(R221P)-pEGFP (D). After 24 h, the cells were fixed and stained with Oil red O (ORO). Immunofluorescent imaging reveals that all ATGL proteins correctly localized to LDs. Quantification of LD number and size per cell was performed using the public-domain Java image-processing program WCIF ImageJ 1.35j (developed by W. Rasband; NIH, Bethesda, Maryland). Fluorescence of EGFP and ATGL-EGFP fusion proteins is shown in green. Magnification: 40×. These data were previously published in a different format [55].