Figure 4.

SYK downregulation reduces the cell viability of SYK expressing neuroblastoma cell lines. SYK-positive SH-SY5Y and LAN-6 cells, as well as SYK-negative SK-N-BE(2) cells, were transfected with 5 pmol SYK specific or scrambled (scr.) siRNA. The medium was replaced after 4 h. After 72 h, the cell viability was evaluated by MTT assay (A). The scr. control was set as 100% viable cells. Data are presented as mean ± SD from three independent experiments. Statistical comparisons were made using two-way ANOVA and a significant effect was observed for the siRNA treatment p < 0.001 and between cell lines p = 0.003. The Dunnett’s multiple comparison test was used to evaluate the difference between scr. siRNA vs. SYK specific siRNA: ** p < 0.01, *** p < 0.001. (B) SYK expression levels were determined by western blot.