Figure 8.

Transfection with an active SYK variant increases the cell viability of neuroblastoma cells independent of endogenous SYK levels. Cells were transfected with expression plasmids encoding different SYK variants and the cell viability was measured after 48 h by MTT assay (A). The empty vector control was set as 100% viable cells. Data are presented as mean ± SD from six independent experiments. Statistical comparisons were made using two-way ANOVA and a difference was observed between the SYK variants and between cell lines p < 0.001. The Dunnett’s multiple comparison test was used to evaluate the difference between empty vector and different SYK variants: * p < 0.05, *** p <0.001. Western blot analysis was used to evaluate the presence of SYK following transfection, ensuring sufficient transfection efficiency (B). SYK wt = SYK wild type, SYK B = short SYK splice variant B, SYK Y130E = constitutive active SYK, SYK K402R = kinase dead SYK, SYK RR42/195KK = SYK with inactive SH2 domains.