Figure 1.

Proliferation and motility of melanoma cells were suppressed with metformin treatment. (A) A2058 cells and (B) A375 cells were treated with 0, 1, 5 and 10 mM metformin for three consecutive days. Cell growth was examined using CellTiter-Glo One solution assay. (C) and (D) Cell migration assay was used to examine A2058 and A375 cells treated with or without metformin (5 mM) for three days (magnification, 100×). Migrating cells were stained with crystal violet solution, and the migration abilities were quantified through measuring three different fields under a phase-contrast microscope.