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. 2019 Feb 11;11(2):209. doi: 10.3390/cancers11020209

Figure 8.

Figure 8

Knockdown of EFEMP1 and SCAMP3 expression significantly inhibited melanoma cell growth. (A) and (B): After siRNA transfection, the expression levels of EFEMP1and SCAMP3 were examined through real-time PCR and Western blotting. (C) and (D): Colony formation assay was examined in A2058 cells with EFEMP1 and SCAMP3 knockdown; and the relative colony formation ability was quantified using OD595 nm. (E) and (F): Cell proliferation was assessed in A2058 cells with EFEMP1 and SCAMP3 knockdown using the CellTiter-Glo One solution assay.