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. 2019 Feb 8;11(9):8749–8762. doi: 10.1021/acsami.8b19094

Figure 3.

Figure 3

Cell proliferation and survival of MSCs in CMCh-ACP hybrid gel in vitro. (A,B) The MSC iMADs preinfected with AdR-GLuc were mixed with CMCh-ACP hybrid gel, seeded into 12 well plates, and grown with complete DMEM (B). RFP signal was detected at 1, 3, 6, 9, 12, and 15 days. At the 15th day, another dose of Ad-GLuc was added to each well to reinfect iMADs in CMCh-ACP hybrid gel; the RFP signal was recorded three days later (day 18). (C) Gaussia luciferase activity assay. At the indicated time points, an aliquot of the culture media from AdR-GLuc-transduced iMADs cultured in CMCh-ACP hybrid gel was collected for GLuc activity analysis. Each assay condition was done in triplicate. “**” p < 0.01, compared with the GLuc activity at day 3.