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. 2019 Feb 8;11(9):8749–8762. doi: 10.1021/acsami.8b19094

Figure 7.

Figure 7

CMCh-ACP hybrid gel acts synergistically with BMP9 and induces more uniformly mineralized ectopic bone formation. (A) Representative images of the subcutaneous masses retrieved at 6 weeks after injection (a). Vascular ingrowth was easily detected inside and outside of the masses retrieved from the BMP9 + CMCh-ACP group. No detectable masses were retrieved from the GFP control or CMCh-ACP only group. The retrieved masses were subjected to micro-CT (μCT) imaging and 3D reconstruction (a). Representative images are shown. The μCT data were further analyzed by using Amir software to determine the mean bone density (b) and trabecular bone area (c). “**” p < 0.01 when compared with that of the BMP9 only group. HU, Hounsfield unit; a.u., arbitrary unit. (B) Hematoxylin and eosin staining and trichrome mineralization staining of the retrieved bone masses from the BMP9 only groups (a) and the BMP9 + CMCh-ACP hybrid gel groups (b) at weeks 4, 6, and 8. Representative images are shown.