Figure 2.

Flow cytometry analysis of the Cy5.5-positive cell population 4 h after treatment of Cy5.5-NP or Cy5.5-CD38-NP. Anti-CD38-conjugated NP exhibits improved cellular uptake of NP by multiple myeloma (MM) cells. Cy5.5 was chemically conjugated to the core of NP. The gated area was defined using the cells without NP treatment. The representative dot plot from a triplicate experiment is shown. The error values represent the standard deviation from the triplicate experiment. A non-MM cell line, SupM2, was included for comparison. The fold change in cell uptake was calculated by dividing the percentage of Cy5.5-positive cells with Cy5.5-CD38-NP treatment by that with Cy5.5-NP treatment. The error bar represents standard deviation from a triplicate experiment; * p < 0.05, via Student’s t-test.