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. 2019 Feb 20;11(2):248. doi: 10.3390/cancers11020248

Figure 3.

Figure 3

CD38-S3I-NP induces cytotoxicity and inhibits STAT3 activity in MM cells. (A) U266 and RPMI8226 cells were then treated with S3I-NP, CD38-S3I-NP, or S3I-NP with free CD38 antibody at a concentration which is equivalent to CD38-S3I-NP (1.4 mg/mL) with the presence of IL6 (2 ng/mL) for 24 and 48 h. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay in triplicate; * p < 0.05, via Student’s t-test. (B) Western blot analysis of STAT3 and pSTAT3 levels in U266 and RPMI8226 cells treated with S3I-NP or CD38-S3I-NP with the presence of IL6 (2 ng/mL) for 24 h. β-actin was blotted as a loading control.