Table 2.
Oligonucleotide primers used for the study of gene expression of selected targets by quantitative real time PCR.
| Target | Primer sequence (5′–3′) | Annealing temperature, °C | PCR product size, bp | GenBank accession No. |
|---|---|---|---|---|
| GAPDH | F:GCTGAATGGGAAGCTTACTG R: AAGGTGGAGGAATGGCTG |
60 | 216 | NM_204305.1 |
| ZO-1 | F:TAAAGCCATTCCTGTAAGCC R: GTTTCACCTTTCTCTTTGTCC |
60 | 243 | XM_015278981.1 |
| ZO-2 | F: GGCAAATCATTGAGCAGGA R: ATTGATGGTGGCTGTAAAGAG |
60 | 239 | XM_015280247.1 |
| CLDN1 | F: CTGATTGCTTCCAACCAG R: CAGGTCAAACAGAGGTACAAG |
59 | 140 | NM_001013611.2 |
| CLDN5 | F: CATCACTTCTCCTTCGTCAGC R: GCACAAAGATCTCCCAGGTC |
59 | 111 | NM_204201.1 |
| OCLN | F: TCATCGCCTCCATCGTCTAC R: TCTTACTGCGCGTCTTCTGG |
62 | 240 | NM_205128.1 |
| TLR2 | F: CTTGGAGATCAGAGTTTGGA R: ATTTGGGAATTTGAGTGCTG |
62 | 238 | XM_015301380.1 |
| TLR4 | F: GTCTCTCCTTCCTTACCTGCTGTTC R: AGGAGGAGAAAGACAGGGTAGGTG |
65 | 187 | NM_001030693.1 |
| NF-κB | F: TGTGGTTGTCAGGATGGTC R: GGTCTGGTAAAGGTCATTTCTC |
62 | 273 | XM_015285418.1 |
| MUC2 | F: GCTGATTGTCACTCACGCCTT R: ATCTGCCTGAATCACAGGTGC |
62 | 442 | XM_421035 |
| sIgA | F: GTCACCGTCACCTGGACTACA R: ACCGATGGTCTCCTTCACATC |
59 | 192 | S40610 |
GAPDH = glyceraldehyde 3-phosphate dehydrogenase; ZO-1, -2 = zona occludens 1, 2; CLDN1, 5 = claudins 1, 5; OCLN = occluding; NF-κB = nuclear factor kappa light-chain-enhancer of activated B cells; TLR2, 4 = Toll-like receptors 2, 4; MUC2 = mucin 2; sIgΑ = secretory immunoglobulin A.
F: forward, R: reverse.