Table 6.
Cecal digesta microbiota composition of 42-day-old broilers.
| Cecal digesta content (log10 cells/g digesta)1 | Total bacteria | Escherichia coli | Lactobacillus spp. | Bifidobacterium spp. | Bacteroides spp. | Clostridium cluster I | Clostridium cluster IV | Clostridium cluster XIVa |
|---|---|---|---|---|---|---|---|---|
| Type of cereal2 | ||||||||
| Maize (M) | 10.09A | 8.16 | 7.40 | 5.26B | 8.01 | 7.89a | 9.25A | 9.74A |
| Wheat (W) | 9.85B | 8.42 | 7.65 | 6.73A | 8.04 | 7.62b | 8.73B | 9.56B |
| PFA supplementation, mg/kg diet3 | ||||||||
| 0 | 9.86 | 8.21 | 7.45 | 6.08 | 7.85 | 7.73 | 8.90 | 9.56 |
| 100 | 10.04 | 8.29 | 7.48 | 5.86 | 8.14 | 7.66 | 9.03 | 9.71 |
| 150 | 10.01 | 8.37 | 7.66 | 6.06 | 8.08 | 7.86 | 9.04 | 9.68 |
| Interaction (treatments)4 | ||||||||
| M0 | 10.01 | 8.04 | 7.49 | 5.48 | 7.91bc | 7.99 | 9.36a | 9.72 |
| M100 | 10.22 | 8.08 | 7.27 | 5.18 | 8.25ab | 7.86 | 9.31a | 9.79 |
| M150 | 10.03 | 8.37 | 7.46 | 5.13 | 7.86c | 7.81 | 9.09ab | 9.70 |
| W0 | 9.70 | 8.39 | 7.41 | 6.68 | 7.79c | 7.48 | 8.44c | 9.40 |
| W100 | 9.85 | 8.49 | 7.69 | 6.53 | 8.03abc | 7.46 | 8.76bc | 9.63 |
| W150 | 10.00 | 8.38 | 7.86 | 6.99 | 8.31a | 7.91 | 9.00ab | 9.65 |
| SEM | 0.089 | 0.219 | 0.182 | 0.193 | 0.123 | 0.132 | 0.157 | 0.064 |
| P-values | ||||||||
| PCT | 0.004 | 0.163 | 0.109 | <0.001 | 0.719 | 0.019 | <0.001 | 0.003 |
| PPFA | 0.110 | 0.766 | 0.484 | 0.455 | 0.063 | 0.344 | 0.593 | 0.067 |
| PCT × PFA | 0.132 | 0.628 | 0.327 | 0.220 | 0.025 | 0.069 | 0.048 | 0.122 |
M0 = maize-soy bean meal (SBM) basal diet with no other additions; M100 = maize-SBM basal diet with addition of 100 mg PFA/kg diet; M150 = maize-SBM basal diet with addition of 150 mg PFA/kg diet; W0 = wheat-SBM basal diet with no other additions; W100 = wheat-SBM basal diet with addition of 100 mg PFA/kg diet; W150 = maize-SBM basal diet with addition of 150 mg PFA/kg diet; SEM = pooled standard error of means; CT = cereal type; PFA = phytogenic feed additive.
a b Within a column, means with different superscripts differ at P < 0.05.
A, B, C Within a column, means with different superscripts differ at P < 0.01.
All microbial cfu data were transformed to respective log10 values before being analyzed.
Basal diets based on maize (M) or wheat (W). Data shown per CT represent treatment means from 15 broilers (e.g. 5 from treatment M0 + 5 from treatment M100 + 5 from treatment M150).
Phytogenic supplementation (0, 100 and 150 mg/kg diet). Data shown for PFA represent means from 10 replicate pens (e.g. 5 from treatment M0 + 5 from treatment W0).
Interaction means (treatments) for 5 battery cages per treatment.