Table 1.
The list of antibodies used for fluorescent multiplex immunohistochemistry analysis.
| Figure | Antibody | Clone (Host)/Company | Dilution | Incubation | TSA Dyes |
|---|---|---|---|---|---|
| 2 | CD8 | C8/144B (mouse)/Nichirei | undiluted | 60 min | 520 |
| pan-Cytokeratin | AE1/AE3 + 5D3 (mouse)/abcam | 1:200 | 60 min | 570 | |
| PD-L1 | E1L3N (rabbit)/Cell Signaling | 1:100 | 60 min | 650 | |
| 3 | CD20 | L26 (mouse)/abcam | 1:50 | 60 min | 520 |
| pan-Cytokeratin | AE1/AE3 + 5D3 (mouse)/abcam | 1:200 | 60 min | 570 | |
| 4 | CD3 | SP7 (rabbit)/abcam | 1:100 | 60 min | 520 |
| FOXP3 | 236A/E7 (mouse)/abcam | 1:100 | 60 min | 570 | |
| pan-Cytokeratin | AE1/AE3 + 5D3 (mouse)/abcam | 1:200 | 60 min | 650 |