A, R273H mutation in p53. Codon 273 of p53 RNA was CAU, rather CGU, in TP53-Dox cells. B, Cell responses to doxorubicin. After pretreatments with siMETTL3 or NPC for 3 days, SW48-Dox (wild-type p53) and TP53-Dox (p53/R273H+) cells were treated with doxorubicin (Dox) combined with these agents for 72 hr. siMETTL3, 100 nM siRNA against human METTL3; siRNA-SC, 100 nM siRNA scrambled control; NPC, 20 nM neplanocin A. *, p<0.001, compared to SW48-Dox cells; ** or #, p<0.001 compared to TP53-Dox cells treated with vehicle or siRNA-SC (not depicted in this graph). C, IC50 values for Dox in cells treated with combinations of NPC, siMETTL3 or PDMP. *, p<0.001, compared to SW48-Dox cells; ** or #, p<0.001 compared to TP53-Dox cells treated with vehicle or siRNA-SC.