Fig. 2.

GSK3β inhibitors decrease the migratory properties of cells with mesenchymal phenotype. a HMLE-Snail, HMLE-Twist, and SUM159 cultures treated with indicated GSK3β inhibitors or DMSO (control) were wounded. After 9 h, the percentage wound closure was determined. Left: representative images of the wound region. Scale bars 100 μm. Right: plots of percentage wound closure in each cell type (n = 3, p values were calculated using Student’s unpaired two-tailed t test). b Cells were stained for FOXC2 (red). The nuclei were labeled with DAPI (blue)