Figure 7.

Neuronal densities were unchanged in areas with atypical astrocytes. Areas with atypical astrocytes are indicated by a solid yellow line. a, In areas with atypical astrocytes, expression of the pan-neuronal marker NeuN was abnormal. Three distinct patterns were seen (rightmost image): those that were consistent in NeuN intensity outside and appeared normal (1), those that had an overall decrease in NeuN expression (2), and those that formed a ring of NeuN where the center of the soma was void of NeuN (3). b, Nissl staining labeled all neurons in areas with abnormal astrocytes at various time points. c, Quantification of neuronal numbers within cortical layers II/III normalized to area size within areas of atypical astrocytes and in peripheral areas. Neuronal densities in immediately adjacent “peripheral” areas were set to 1 and the fold change of densities within areas of loss was computed for each ROI. See Figure 7-1 for breakdown of sample sizes dependent on sex for representative images and detailed numerical data for all statistical group comparisons.