Table 1.
Manufacturer information, working dilutions and resource identification numbers for primary and secondary antibodies used for immunohistochemistry
| Name | Manufacturer | Catalog # | RRID | Species raised in | Monoclonal/polyclonal | Concentration |
|---|---|---|---|---|---|---|
| Primary antibodies | ||||||
| GFP | Aves Lab | GFP-1020 | AB_300798 | Chicken | Polyclonal | 1:1000 |
| GFAP | Millipore | MAB360 | AB_2109815 | Mouse | Monoclonal | 1:1000 |
| GFAP | Dako | Z0334 | AB_10013382 | Rabbit | Polyclonal | 1:1000 |
| GFAP | Abcam | AB4674 | AB_304558 | Chicken | Polyclonal | 1:1000 |
| Vimentin | Millipore | AB5733 | AB_1121377 | Chicken | Polyclonal | 1:1000–1:5000 |
| Nestin | Millipore | MAB353 | AB_94911 | Mouse | Monoclonal | 1:200 |
| Glt1 | Millipore | AB1783 | AB_90949 | Guinea Pig | Polyclonal | 1:1000 |
| Kir4.1 | Alomone | APC035 | AB_2040120 | Rabbit | Polyclonal | 1:400 |
| Cleaved Caspase 3 | Cell Signaling Technology | 9661 | AB_2341188 | Rabbit | Polyclonal | 1:100 |
| S100β | Sigma-Aldrich | S2532 | AB_477499 | Mouse | Monoclonal | 1:1000 |
| S100 | Dako | 0803 | Not assigned | Rabbit | Polyclonal | 1:1000 |
| Iba1 | Wako | 019-9741 | AB_839504 | Rabbit | Polyclonal | 1:1000 |
| NeuN | Millipore | MAB377 | AB_2298772 | Mouse | Monoclonal | 1:200 |
| NeuN | Millipore | ABN78 | AB_10807945 | Rabbit | Polyclonal | 1:500 |
| CD11b | Bio-Rad | MCA74G | AB_321293 | Rat | Monoclonal | 1:500 |
| Ki-67 | Thermo Scientific | RM-9106-S1 | AB_149792 | Rabbit | Monoclonal | 1:100 |
| BrdU | Abcam | AB6326 | AB_305426 | Rat | Monoclonal | 1:500 |
| Anti-human tau | Thermo Scientific | MN1000B | AB_223453 | Mouse | Monoclonal | 1:100 |
| Secondary antibodies | ||||||
| Chicken Alexa Fluor-488 | Jackson Immuno Research | 703-546-155 | AB_2340376 | Donkey | Polyclonal | 1:1000 |
| Chicken Cy3 | Jackson Immuno Research | 703-166-155 | AB_2340364 | Donkey | Polyclonal | 1:1000 |
| Chicken Alexa Fluor-647 | Jackson Immuno Research | 703-606-155 | AB_2340380 | Donkey | Polyclonal | 1:1000 |
| Rabbit Alexa Fluor-488 | Jackson Immuno Research | 111-546-144 | AB_2338057 | Goat | Polyclonal | 1:1000 |
| Rabbit Cy3 | Jackson Immuno Research | 111-166-144 | AB_2338011 | Goat | Polyclonal | 1:1000 |
| Rabbit Alexa Fluor-647 | Jackson Immuno Research | 111-606-144 | AB_2338083 | Goat | Polyclonal | 1:1000 |
| Mouse Alexa Fluor-488 | Jackson Immuno Research | 115-546-003 | AB_2338859 | Goat | Polyclonal | 1:1000 |
| Mouse Cy3 | Jackson Immuno Research | 115-166-003 | AB_2338699 | Goat | Polyclonal | 1:1000 |
| Mouse Alexa Fluor-647 | Jackson Immuno Research | 115-606-003 | AB_2338921 | Goat | Polyclonal | 1:1000 |
| Rat Alexa Fluor-488 | Jackson Immuno Research | 112-546-003 | AB_2338364 | Goat | Polyclonal | 1:1000 |
| Rat Cy3 | Jackson Immuno Research | 112-166-003 | AB_2338253 | Goat | Polyclonal | 1:1000 |
| Rat Alexa Fluor-647 | Jackson Immuno Research | 112-606-003 | AB_2338406 | Goat | Polyclonal | 1:1000 |
| Guinea pig Alexa Fluor-488 | Jackson Immuno Research | 106-546-003 | AB_2337441 | Goat | Polyclonal | 1:1000 |
| Guinea pig Cy3 | Jackson Immuno Research | 106-166-003 | AB_2337426 | Guinea pig Cy3 | Polyclonal | 1:1000 |
| Guinea pig Alexa Fluor-647 | Jackson Immuno Research | 106-606-003 | AB_2337449 | Goat | Polyclonal | 1:1000 |
| Dyes | ||||||
| DAPI | Thermo Fisher | D1306 | AB_2629482 | N/A | N/A | 1:1000 |
| Alexa Fluor-555 cadaverine | Thermo Fisher | A30677 | N/A | N/A | N/A | 0.33 mg/mouse |
| NeuroTrace 530/615 Nissl | Thermo Fisher | N21482 | AB_2620170 | N/A | N/A | 1:100 |
N/A, Not applicable.