Table 2.
Manufacturer information, working dilutions, and resource identification numbers for primary and secondary antibodies used for Western blot
| Name | Manufacturer | Catalog # | RRID | Species raised in | Monoclonal/polyclonal | Concentration |
|---|---|---|---|---|---|---|
| Primary antibodies | ||||||
| GFAP | Dako | Z0334 | AB_10013382 | Rabbit | Polyclonal | 1:5000 |
| Vimentin | Millipore | AB5733 | AB_11212377 | Chicken | Polyclonal | 1:5000 |
| Vimentin | Thermo Fisher | MA3-745 | AB_326285 | Mouse | Monoclonal | 1:5000 |
| Nestin | Abcam | AB6142 | AB_305426 | Mouse | Monoclonal | 1:2500 |
| Cleaved Caspase 3 | Cell Signaling Technology | 9611S | AB_2341188 | Rabbit | Polyclonal | 1:1500 |
| GAPDH | Abcam | AB8245 | AB_2107448 | Mouse | Monoclonal | 1:5000 |
| Secondary antibodies | ||||||
| AzureSpectra goat anti-rabbit IgG IR800 antibody | Azure Biosystems | AC2134 | Not assigned | Goat | Polyclonal | 1:1500 |
| AzureSpectra goat anti-mouse IgG IR700 antibody | Azure Biosystems | AC2129 | Not assigned | Goat | Polyclonal | 1:1500 |
| AzureSpectra goat anti-mouse IgG IR800 antibody | Azure Biosystems | AC2135 | Not assigned | Goat | Polyclonal | 1:1500 |
| AzureSpectra goat anti-rabbit IgG IR700 antibody | Azure Biosystems | AC2128 | Not assigned | Goat | Polyclonal | 1:1500 |
| AzureSpectra goat anti-chicken IgG IR700 antibody | Azure Biosystems | AC2131 | Not assigned | Goat | Polyclonal | 1:1500 |
| Goat anti-rabbit IgG HRP | Santa Cruz Biotechnology | SC2004 | AB_631746 | Goat | Polyclonal | 1:1500 |
| Goat anti-mouse IgG HRP | Santa Cruz Biotechnology | SC2055 | AB_631738 | Goat | Polyclonal | 1:1500 |
| Goat anti-chicken IgG HRP | Santa Cruz Biotechnology | SC2901 | AB_650515 | Goat | Polyclonal | 1:1500 |
| Chemiluminescent substrates | ||||||
| Name | Manufacturer | Catalog # | Development conditions | Concentration | ||
| Clarity Western ECL substrate-peroxide solution | Bio-Rad | 102030831 | 1 min incubation, room temperature | 1:2 | ||
| Clarity Western ECL substrate-luminol/enhancer solution | Bio-Rad | 102030829 | 1 min incubation, room temperature | 1:2 | ||
N/A, Not applicable.