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. 2019 Mar 1;10:134. doi: 10.3389/fendo.2019.00134

Figure 2.

Figure 2

FAM83F overexpression effects on thyroid differentiation genes of PCCL3 cell line. (A) Validation of FAM83F gene expression increase in PCCL3-FAM83F. In this case, data shown as relative expression due to undetectable levels of FAM83F in PCCL3-ϕ by qPCR; Expression levels of (B) Nis; (C): Tg; (D) Tpo; (E) Tshr; (F) Ttf1, and (G) Pax8 in PCCL3-FAM83F cells by qPCR. Data shown as mean ± SD of two independent experiments performed in triplicate normalized by PCCL3-ϕ. *P < 0.05, **P < 0.01 and **P < 0.001. (H) Protein expression of NIS, TTF1 (NKX2-1), and PAX8 analyzed by Western-blotting in PCCL3-FAM83F cells. (I) Transcriptional activity of Nis promoter measured after transfection of a reporter assay with pNis 2.8 luciferase plasmid; and (J) Tg promoter activity measured with pGl-Tg luciferase plasmid into PCCL3-FAM83F cells compared to PCCL3-ϕ. Data shown as mean ± SD of two independent experiments performed in triplicate. **P < 0.01 and ***P < 0.001.