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. 2019 Mar 1;10:134. doi: 10.3389/fendo.2019.00134

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Copyright © 2019 Fuziwara, Saito, Leoni, Waitzberg and Kimura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAM83F overexpression effects on PCCL3 cells biology. (A) Apoptosis levels in response to doxorubicin treatment. PCCL3-FAM83F and PCCL3-ϕ cells were treated with doxorubicin for 24 h and apoptosis was detected as Annexin-V-FITC positivity. Representative results of two independent experiments performed in triplicate. (B) Cell cycle analysis of PCCL3-FAM83F and PCCL3-ϕ cells performed by flow-cytometry with PI staining. ^*P < 0.05, ^**P < 0.01, and^**P < 0.001. (C) PCCL3-FAM83F and PCCL3-ϕ cell viability was measured by MTT-assay. Data shown as mean ± SD of two independent experiments performed in triplicate. (D) Cell migration analysis of PCCL3-FAM83F and PCCL3-ϕ measured by 8 μm-pore transwell assay. Representative image of two independent experiments performed in triplicate. ^*P < 0.05, ^**P < 0.01, and ^**P < 0.001.