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. 2019 Mar 1;10:134. doi: 10.3389/fendo.2019.00134

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Copyright © 2019 Fuziwara, Saito, Leoni, Waitzberg and Kimura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAM83F overexpression effect on TGFβ signaling in PCCL3 cells. (A,B) Responsiveness of TGFβ signaling was analyzed 24-h after the co-transfection of p3TP-lux and 4 × SBE luciferase reporter plasmids with pRl (Renilla luciferase) plasmid and treatment with 1 ng/ml TGFβ in PCCL3-FAM83F and PCCL3-ϕ cells. (C) Cell counting assay in response to rTGFβ1. Cells were seeded at the density of 5 × 10⁴ cell/ well in 6-well plate and treated with 1 ng/ml of rTGFβ1 for 24–72 h and counted in a Guava minicytometer (Millipore). Data shown as mean ± SD of two independent experiments performed in triplicate. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001.