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Effect of PLX3397 in ATLL cell lines. ATL-T and ED cell lines were cultured with or without the M-CSFR inhibitor, PLX3397, for 2 days, and cell viability was tested with the WST assay (A). IC for cleaved caspase-3 was performed using ATL-T cells cultured with PLX3397 (100 μM), and apoptotic cells positive for cleaved caspase-3 were counted (n = 3) (B). Western blot analysis of caspase-3, cleaved caspase-3, and PARP using cell lysates of ATL-T cells treated with PLX3397 (100 μM) (C).
