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. 2019 Mar 5;7:e6565. doi: 10.7717/peerj.6565

Figure 3. Effects of baicalin on Nrf-2 and catalase in bovine MEC.

Figure 3

MEC were cultured for 24 h in medium without (control) or with different concentrations of baicalin (5, 10 and 100 µg/mL). The cells were lysed in MPER buffer for total protein extraction, and 15 μg of protein was analyzed using electrophoresis and Western blotting (Catalase (A) and Nrf 2 (C) antibodies). Each band was quantified using a molecular imager and for each treatment, Catalase (B, 64 kDa) and Nrf 2 (D, 61 kDa) data were normalized using actin (40 kDa) data. Experiments have been performed four times. *p < 0.05 versus control.