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. 2019 Mar 5;12:1825–1839. doi: 10.2147/OTT.S176582

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© 2019 Zhou et al. This work is published and licensed by Dove Medical Press Limited

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Silencing of USP4 promoted cell apoptosis in GBM cell lines.

Notes: (A) Cell apoptosis was detected by flow cytometry using annexin-V/PI kit in shUSP4- or NC-transduced U87 and T98G cells. (B, C) Real-time PCR (B) and Western blot (C) were used to measure the expression level of PCNA, Bcl-2, Bax and cleaved caspase3 in shUSP4- or NC-transduced U87 and T98G cells. ***P<0.001 compared to NC.

Abbreviations: GBM, glioblastoma multiforme; NC, transduced with negative control; shUSP4, transduced with shUSP4 lentivirus; USP4, Ubiquitin-specific protease 4; WT, wild type cells.

Silencing of USP4 promoted cell apoptosis in GBM cell lines.

Notes: (A) Cell apoptosis was detected by flow cytometry using annexin-V/PI kit in shUSP4- or NC-transduced U87 and T98G cells. (B, C) Real-time PCR (B) and Western blot (C) were used to measure the expression level of PCNA, Bcl-2, Bax and cleaved caspase3 in shUSP4- or NC-transduced U87 and T98G cells. ***P<0.001 compared to NC.

Abbreviations: GBM, glioblastoma multiforme; NC, transduced with negative control; shUSP4, transduced with shUSP4 lentivirus; USP4, Ubiquitin-specific protease 4; WT, wild type cells.