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Detection of O2•− in frozen aortic section from WT and AMPKα2 KO mice using DHE. Fluorescent micrographs of frozen sections of aorta from WT mice and AMPKα2 KO mice are obtained after staining with DHE (5 μM) for 20 min. AMPKα2 deficiency increased O2•− production in both aortic endothelial cells (EC) and vascular smooth muscle cell (VSMC).
