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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Mol Cell. 2018 Nov 8;73(1):73–83.e6. doi: 10.1016/j.molcel.2018.10.006

Figure 3. The ZMET2 CD recognizes H3K9me in the binding step and the BAH domain recognizes H3K9me in the catalytic step.

Figure 3.

(A) WT, F441A and W224L ZMET2 affinity for fluorescein-labeled H3 tail peptides measured by fluorescence polarization. (For these experiments, n=2).

(B) WT, F441A and W224L ZMET2 affinity for WT and H3Kc9me3 mononucleosomes measured by fluorescence polarization. Experiments with WT and F441A (CDx) ZMET2 were performed in duplicate (n=2). Experiments with W224L (BAHx) were performed in quadruplicate (n=4).

(C) DNA methyltransferase activity for WT, F441A and W224L ZMET2 on WT and H3Kc9me3 dinucleosomes. Reactions were conducted under single-turnover conditions in which the concentration of each ZMET2 protein was in excess and saturating over the dinucleosome concentration. (For these experiments, n=2).