REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Rosetta (DE3)	Novagen	70954
BL21 (DE3) pLysS	Promega	L1195
Dam-/dcm-	NEB	C2925I
Chemicals, Peptides, and Recombinant Proteins
DNase I	Roche	10104159001
(2-Bromoethyl)trimethylammonium bromide	Aldrich	117196
(2-Bromoethyl)trimethylammonium bromide	Aldrich	117196
S-[methyl-3H]-Adenosyl-L-Methionine	Perkin Elmer	NET155H250UC
S-(5’-Adenosyl)-L-Methionine Iodide	Sigma	A4377
Proteinase K	Roche	3115887001
EZ DNA Methylation Kit	Zymo	D5001
H3K9me peptide (aa 1–32) ARTKQTARKSTGGKAPRKQLATKAARKSAPAT	CPC Scientific	N/A
H3K9me2 peptide (aa 1–32) ARTKQTAR(Kme2)STGGKAPRKQLATKAARKSAPAT	CPC Scientific	N/A
H3K9me3 peptide (aa 1–32) ARTKQTAR(Kme3)STGGKAPRKQLATKAARKSAPAT	CPC Scientific	N/A
H3Kc9me3 peptide (aa 1–32) ARTKQTARCSTGGKAPRKQLATKAARKSAPAT	CPC Scientific	N/A
DraNI	NEB	R3510L
Deposited Data
Negative stain 3D reconstruction of full-length ZMET2 bound to H3Kc9me3 601 dinucleosome with 20 bp DNA linker	This paper	EMD-9127
Oligonucleotides
unmethylated 38 bp oligo AACGCAGCATGCGCTGCTAGC GCAGCTAGCGCTGCATG	This paper	N/A
hemimethylated 38 bp oligo (annealed with unmethylated complement) AACG(meC)AGCATGCG(meC)TGCTAGCG(meC)AGCTAGCG(meCTG)CATG	This paper	N/A
601 mononucleosome DNA sequence CTGGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCA CCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGAT TACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTGT	This paper	N/A
601 dinucleosome DNA sequence ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCA CCGCTTAAACGCACGTACGGATTCTCCCCCGCGTTTTAACCGCCAAGGGGATT ACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCGATGGTTAACGGAT CTGGCCGCCATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACA GCTCTAGCACCGCTTAAACGCACGTACGGATTCTCCCCCGCGTTTTAACCGCC AAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCGAT	This paper	N/A
5S dinucleosome DNA sequence ATCCCCGACCCTGCTTGGCTTCCGAGATCAGACGATTTCGGGCACTTTCAGGG TGGTATGGCCGTAGGCGAGCACAAGGCTGACTTTTCCTCCCCTTGTGCTGCCT TCTGGGGGGGGCCCAGCCGGATCCCCGGGCGAGCTCGAATTGGTTAACGGA TCTGGCCGCCGGCCCGACCCTGCTTGGCTTCCGAGATCAGACGATTTCGGGC ACTTTCAGGGTGGTATGGCCGTAGGCGAGCACAAGGCTGACTTTTCCTCCCCT TGTGCTGCCTTCTGGGGGGGGCCCAGCCGGATCCCCGGGCGAGCTCGAAGA T	This paper	N/A
Recombinant DNA
Pet3a_H2A (Xenopus laevis)	Canzio et al., 2011	N/A
Pet3a_H2B (Xenopus laevis)	Canzio et al., 2011	N/A
Pet3a_H3 (Xenopus laevis)	Canzio et al., 2011	N/A
Pet3a_H4 (Xenopus laevis)	Canzio et al., 2011	N/A
pUC157 2_167 601 dinucleosome (20 bp linker)	Dr. Karim Armache	N/A
pUC157 2_157 601 dinucleosome (10 bp linker)	This paper	N/A
pUC157 2_177 601 dinucleosome (30 bp linker, one CHG site)	This paper	N/A
pUC157 2_177 601 dinucleosome (30 bp linker, two CHG sites)	This paper	N/A
pUC157 2_187 601 dinucleosome (40 bp linker)	This paper	N/A
pSUMO full-length ZMET2 (codon optimized for bacterial expression)	This paper	N/A
Software and Algorithms
Prism	Graphpad	N/A
ASTRA	Wyatt	N/A
FastQC (v0.11.3)	http://www.bioinformatics.babraham.ac.uk/projects/fastqc	N/A
PANDAseq (v2.10)	Masella et al., 2012	N/A
BSseeker2 (v2.0.9)	W. Guo et al., 2013	N/A
cryoSPARC	Punjani et al., 2017
SerialEM	Mastronarde, 2005
RELION	Scheres, 2012
GCTF	K. Zhang, 2016