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. Author manuscript; available in PMC: 2019 Mar 8.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2018 Jun 28;1865(9):1341–1355. doi: 10.1016/j.bbamcr.2018.06.013

Fig. 6.

Fig. 6.

Dynamic interaction of CaV1.2 vesicles with the plasma membrane requires an intact cytoskeleton. Representative dual-color spinning-disk 3D images of CaV1.2-RFP before (i) and after (ii and iii) application of the FM 1–43 dye in vehicle (A) or 10 μM nocodazole + cytocholasin-D (B) -treated cells. White arrows in panel A point to numerous FM 1–43 dye-loaded CaV1.2-RFP-containing vesicles, suggesting active communication between the CaV1.2-RFP structures and the plasma membrane. Plot depicting the time lapse of normalized fluorescence intensity (AU) for the FM 1–43 dye in the perimeter (p) and intracellular (i) side of the cell before and after its application in (C) vehicle or (D) 10 μM nocodazole + cytochalasin-D (n = 6 cells per condition) -treated cells. Data are shown as mean ± SEM.