Figure 6.

Role of Sox17 (SRY [sex-determining region Y]-box 17) expression on blood-brain barrier (BBB)-related genes at P5 and P9. A, Scheme of tamoxifen injection in the pups at P1 (black arrow) and analysis of endothelial cells isolated from brains at P5 and P9 (red arrows). B, Volcano plots are used to display the magnitude of the differential expression between wild-type (wt) and Sox17^iECKO either at P5 (left) or at P9 (right; n=3 wt and 3 Sox17^iECKO for each time point). Each dot represents 1 gene that has detectable expression both in wt and Sox17^iECKO cells. Black dots represent genes that are not significantly different (adjusted P>0.05). Significant differentially expressed genes (DEGs; adjusted P≤0.05) are colored either in red (Log2FC≥0.4) or in blue (Log2FC<0.4). The number of DEGs in Sox17^iECKO are reported in the table on top of the Volcano plots. C, Description of thresholds and colors used in D–G. D, Selected angiogenesis and Wnt/β-cat (β-catenin) related genes, differentially expressed, either up or down, in Sox17^iECKO. E–G, Regulation of the expression of gene relevant for key BBB features (cell to cell junction [E], transcellular permeability [E], transporters [F], and extracellular matrix [G]), as in Figure 5, modulated by the inactivation of Sox17 expression. The genes that do not have any expression changes are displayed by white box. FC indicates fold change.