RPL Peak Genomic Location (mm9)	Distance relative to (CAGAGG)n repeat region	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
Chr7: 35943530 – 35946581	20 kb 5'	GCAAGCCATGGAACTCATCTA	TGGCATGAAGACACCAAGAG
	1,260 bp 5'	CCCTGGATGTGGTTGTCATTA	GGAACAGAACTGCTCACAAATG
	220 bp 5'	ACACCCAGTGAACCAAAGTATAG	CCACCAGTCTTGACTTACTCAC
	230 bp 3'	GACAAGTGCCTGAGGGATAAA	ACTCAGGAGACAGAGGGTTAT
	530 bp 3'	AGAATCCTCGGGTGGAAATG	CAGTGGCTCCTGTTGTGTAT
	20 kb 3'	GCTGAGGCAGGGATAGATTTG	AGGCTTGAGAAGAGTTGAAGATAAG
Chr6: 87722438 – 87728725	20 kb 5'	CCGACTGACCTGTGCTATTT	CATGTGGTTGCTGGGATTTG
	1,450 bp 5'	GTGATTGCCACCAAACCTAATG	CTGGGAAAGGCAGAGAACTAC
	285 bp 5'	CCCAGCACTTGGTAAGTAGAG	AACTCTCGCAGCCGTTTAT
	970 bp 3'	GCCCAAGGCTCTACCATAAA	GCCTGGAACCTACCTTCATAC
	20 kb 3'	CACAGACACGCACACATAGA	TGCTAGGGCCTGAGAATAGA
Chr11:5641800–5644000	20 kb 5'	TCCTTCCATTTCTCTCCATTCC	CACGGTCAATTCGGACTTCT
	430 bp 5'	TGGGCTCTGCCTACTTACTA	GCCCTCAGAGAAGAAGAGAATG
	115 bp 5'	GGCTGGATTCAAGGCAGTAA	CCTCCCAAGTGCTGAGATTAAA
	575 bp 3'	TGGGCAGCGAAGATGAAAT	GCCAAGCCACTCCCTTATT
	20 kb 3'	GTGCAGAGACTATGGAGGAATG	GCAAAGGATCCTGGGTTGTA
	20 kb 3'	CCTTTGCAGGAAGTAGGTCAA	AGAGATGCCAGGAGCAAATC
RPL Peak Genomic Location (mm9)	Distance relative to (CAGG)n repeat region	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
Chr9:121838100–121841700	20 kb 5'	GTGACTTTATCTCCACCGTACTC	CTACTGGAGTGAACCTGGAATG
	460 bp 5'	GCAGCTTGGCTCTGTATCTAT	GTGTGTGTGTTCAGAGGTCTAT
	165 bp 5'	CCTGTCTCTGACACAATGTACTC	CCCAATACACCGCTCCTTT
	230 bp 3'	GCCTCTAGTGATACATCTCCTCTA	GATAAGGCCATCTGCAGTGAT
	360 bp 3'	CAAAGTGCCTCTCGCCTAAA	GCCTGGCTTCACAAGGATAG
	20 kb 3'	CCTTTGCAGGAAGTAGGTCAA	AGAGATGCCAGGAGCAAATC
RPL Peak Genomic Location (mm9)	Distance relative to (CACAG)n repeat region	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
Chr17: 13737678–13751955	20 kb 5’	CTTTCTGTGTCCGTGTCCTATC	CACCAACCGTAGAATGGGTATC
	1,085 bp 5’	TTCAAGGTGAGAGAGATGGATTG	GCCTTCTCCTGTGCTGTATT
	600 bp 5’	GAAGTATCAGGCAGCAAGGAA	CTGACTTTGACGGCAGGATAA
	0 bp 3’	GCTGTCTCTGCTGTCTGTAATG	GCTACTCTTGTCCCATGTTTCC
	500 bp 3’	GCCTCCAGGTTGCCTAAATA	GCTCTCTGTTGCTATGGTGAA
	1,400 bp 3’	CCCGCTAATTCCTCTGAAGTC	TCTTCCCTGACTGGTCCTATT
Chr6: 29694103–29706050	30 kb 5’	GTCTTCTGTAGGACCAGAGTTTG	GAAGTCCAGAAGAGAGCATCAG
	20 kb 5’	GCCACAGGTATTCATCCAAGA	GCCTTTATCGCTCAGGTATGT
	1,200 bp 5’	GCCAGGACTACACAGAGAAAC	TGGAGATCCAACTCCCTCTT
	170 bp 5’	ATCCACCACCTCCAACAAG	GAATTCCACCCATAGGCTCATA
	190 bp 3’	AGGTGCAGTGACATGAGTTC	CCTATGGGATCCTGCTATCTCTA
	400 bp 3’	CCCTAGATGGCTCCTTAATTGT	GAACTCACAGAGATCCACCTTC
	1,015 bp 3’	CCTCTGCTCACTCCAATCAAT	TGAGATTTGACGCCCTCTTC
	20 kb 3’	AGTCTTCTCCCACCCTTGA	GGCGCTACCTCCATGTTATT
	30 kb 3’	CCCTAACTGGTCTGGGATTTG	CACCTTTCACCACAGCACTA
I-PpoI site in rDNA sequence	Distance relative to I-PpoI site	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
	11.5 kb 3'	CACGCTGTCCTTTCCCTATT	GACAGACCCAAGCCAGTAAA
	7 kb 3'	CTGAGAAACGGCTACCACATC	GCCTCGAAAGAGTCCTGTATTG
	780 bp 3'	ACAGCCTCTGGCATGTTG	GCCAATCCTTATCCCGAAGTTA
	70 bp 3'	CTAGCAGCCGACTTAGAACTG	CAGAAATCACATCGCGTCAAC
	60 bp 5'	CCTACCTACTATCCAGCGAAAC	CTACACCTCTCATGTCTCTTCAC
	120 bp 5'	GGGAAAGAAGACCCTGTTGAG	GGCCTCCCACTTATTCTACAC
	750 bp 5'	GAACGTGAGCTGGGTTTAGA	CTCTCGTACTGAGCAGGATTAC
	20 kb 5'	GAAACCAAAGCGACCTGAAAC	CAGCCATCTTGTCTGCTAACT
HFUGW vector	Distance relative to insert site	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
	1,764 bp 5’	TGTACTGGGTCTCTCTGGTTAG	AGCACTCAAGGCAAGCTTTA
	909 bp 5’	GCTTTGTTCCTTGGGTTCTTG	TTCTGCTGCTGCACTATACC
	338 bp 5’	GAGTTAGGCAGGGATATTCACC	TCTCTCTCTCCACCTTCTTCTT
	153 bp 3’	CATAAGACTCGGCCTTAGAACC	GTTCCGCTCTCTGGAAAGAA
	959 bp 3’	CTAGGGTAGGCTCTCCTGAAT	ACCGGAGCTTCAGCTACTTA
Break Peak Genomic Location (hg19)	Distance relative to repeat region	Forward Primer (5' -> 3')	Reverse Primer (5' -> 3')
Chr12:121692970–121693250	730 bp 5’	AGGCAACAGGGCAAGAAT	GAGATCAGCCAGTACACATCAG
	220 bp 5’	AGGCAGACCCACAATCTAATC	CAGGAAGCATCCAGATAGGAAA
	30 kb 3’	CAGGGACATGGACATGACTAAC	GGCCTTGGGACAGAAGAAATA
Chr6:55491300–55494100	20 kb 5’	GTCTTCAGCTGTCAGTCTCTTC	GCTGAGATGCTGGTCCTTTAT
	230 bp 5’	TCTAATCAGCCTCCAGGGAATA	GAAGCATCCAGCACAAGAGA
	20 kb 3’	GCACCCACTAATGTGTCATCTA	GAGATCACTTGGACACAGGAAG
Chr6:81329562–81332945	30 kb 5’	AACTCACTCGTTACCACAAGG	GTCTCCACCCAAATCTCATCTC
	715 bp 5’	GGGCAAGAGTCTCATGTGTAT	CTCAGCCTTTCTGGTCTTGT
	520 bp 3’	TCACCAACACCACTTCCTTATC	CTCCCAGTCATTGTGCTCTC