Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (S. cerevisiae) | See Supplementary file 3 | |||
| Strain, strain background (S. cerevisiae) |
S288c | |||
| Genetic reagent (S. cerevisiae) |
See Supplementary file 3 | |||
| Antibody | anti-FLAG-HRP (mouse monoclonal) | Sigma | A8592 | (1:1000) |
| Antibody | anti-PGK1 (mouse monoclonal) | Invitrogen | 459250 | (1:5000) |
| Antibody | goat anti-mouse-IgG-HRP (rabbit polyclonal) | Abcam | Ab97046 | (1:10000) |
| Recombinant DNA reagent |
See Supplementary file 4 | |||
| Cell line (T. ni) | High Five cells; Trichoplusia ni |
ThermoFisher | B85502 | Harrison lab stock |
| Cell line (E. coli) | Rosetta 2(DE3)pLysS; E. coli | EMD Millipore | 71403 | Harrison lab stock |
| Chemical compound, drug |
Glutaraldehyde | Sigma | G4004 | |
| Software, algorithm | UCSF Image4 | Li et al., 2015 | ||
| Software, algorithm | SerialEM | Mastronarde, 2005 | ||
| Software, algorithm | MotionCor2 (v1.1.0) | Zheng et al., 2017 | ||
| Software, algorithm | CTFFIND4 (v4.1.8) | Rohou and Grigorieff, 2015 | ||
| Software, algorithm | Relion (v2.1) | Kimanius et al., 2016 | ||
| Software, algorithm | Eman2 (v2.22); e2initialmodel.py | Tang et al., 2007 | ||
| Software, algorithm | ResMap (v1.1.4) | Kucukelbir et al., 2014 | ||
| Software, algorithm | PyMol (v2.1.0) | Schrödinger, LLC | ||
| oftware, algorithm | Chimera (v1.11.2) | Pettersen et al., 2004 | ||
| Software, algorithm | Coot (v0.8.8) | Emsley et al., 2010 | ||
| Software, algorithm | Phenix (v1.13) | Afonine et al., 2018 | ||
| Software, algorithm | TrackMate (v3.0.0) | Tinevez et al., 2017 | ||
| Software, algorithm | MAFFT | Katoh et al., 2017 | ||
| Software, algorithm | JalView | Waterhouse et al., 2009 | ||
| Software, algorithm | Phyre2 | Kelley et al., 2015 | ||
| Software, algorithm | Fiji | Schindelin et al., 2012 | ||
| Software, algorithm | python 2.7.2 | www.python.org | ||
| Other | C-flat | Electron Microscopy Sciences |
CF-1.2/1.3–3C | holey carbon grids |