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. 2019 Mar 8;14(3):e0211339. doi: 10.1371/journal.pone.0211339

Fig 1. Study overview.

Fig 1

Cells: fibroblasts, mesenchymal stem cells, smooth muscle cells, or endothelial cells were cultured respectively. Multicellular spheroids were created using mixed cell suspensions, and the artificial esophagus was then constructed with bio-3D printing using those spheroids. The artificial esophagus was matured in a bioreactor for a total of 4 weeks. Finally, esophageal transplantation of the artificial esophagus was performed.