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. Author manuscript; available in PMC: 2020 Mar 7.
Published in final edited form as: Mol Cell. 2018 Dec 4;73(5):1001–1014.e8. doi: 10.1016/j.molcel.2018.11.028

Fig 2– DG accumulation induced by αS expression is toxic and causes a trafficking defect.

Fig 2–

A) Neutral lipid profiles of human αS expression in wt and dga1Δ lro1Δ yeast 6 h post induction. B) αS-induced ER accumulation of CPY is exacerbated in dga1Δ lro1Δ vs wt. CPY Immunoblot (ImageJ quantified). n=3. p=0.01, t-test. C) Deletion of lipases TGL3 and TGL4 rescues αS toxicity. D) Neutral lipid profiles of human αS expression in wt and tgl3Δ tgl4Δ yeast 12 h post induction. E) αS-induced ER accumulation of CPY is reduced in the tgl3Δ tgl4Δ strain (ImageJ quantified). n=3.p=0.007,t-test. F) Choline addition rescues αS toxicity. G) αS-induced ER accumulation of CPY is alleviated upon 0.5 mM choline addition (ImageJ quantified). n=3. p=0.002, t-test.