Fig.2.

a Observed distribution of X-chromosome nuclear content of the hepatocytes in the aggregated slides of female recipients, regardless of the sex of the donor. The predicted distribution is the best-fit model assuming equal probability of detecting any given X signal, optimizing the probability of detection (pX) and the tetraploid frequency (pTET) to maximize the chi-square statistic. The best fit result was pX=0.507. b The hypothesis that the Ycontaining nuclei in female recipients of male donors also followed an equal probability model was tested. With the best fit pX (as above) as the input to the binomial best-fit model, the chromosomal content was predicted. The observed and the predicted were compared (the outlined XY not included), with chi-squared p=.015 A modified model was developed, assuming X is “clumpy” in tetraploids. The outlined Y–only data was added to the original XY data (indicated as outlined XY data) and the original Y–only data was not included in the modified model. The observed and the predicted were compared, with chi-squared p=.6 2c The male recipient of a male transplant compared the observed and predicted sex chromatin, with chi-squared p=.6.